Pegylation of Peptides the Case for Peg Conjugation

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Chemistry for peptide and protein PEGylation.

Poly(ethylene glycol) (PEG) is a highly investigated polymer for the covalent modification of biological macromolecules and surfaces for many pharmaceutical and biotechnical applications. In the modification of biological macromolecules, peptides and proteins are of extreme importance. Reasons for PEGylation (i.e. the covalent attachment of PEG) of peptides and proteins are numerous and include...

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Biodrugs 2008; 22 (5): 315-329

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Ijprbs 202

The paper discusses general problems in using PEG for conjugation to high or low molecular weight molecules. Poly (ethylene glycol) (PEG) is a highly investigated polymer for the covalent modification of biological macromolecules and surfaces for many pharmaceutical and biotechnical applications. PEGylation is the process of covalent attachment of polyethylene glycol (PEG) polymer chains to ano...

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Peptide and protein PEGylation: a review of problems and solutions.

The paper discusses general problems in using PEG for conjugation to high or low molecular weight molecules. Methods of binding PEG to different functional groups in macromolecules is reported together with their eventual limitations. Problems encountered in conjugation, such as the evaluation of the number of PEG chains bound, the localisation of the site of conjugation in polypeptides and the...

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Monitoring Peptide PEGylation by HPLC with Charged Aerosol Detection

Purpose: The goal of this work was to develop a fast and accurate method to monitor PEGylation reactions by using HPLC with complementary UV/Vis and charged aerosol detection. It is an increasingly common practice to modify biotherapeutic peptides, proteins and small molecules by attaching chemical groups designed to enhance properties such as bioavailability, potency or stability. One example ...

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تاریخ انتشار 2015